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目的 :观察中等强度 (5 5 %～ 75 %V0 2max)耐力运动锻炼对正常及肺气肿大鼠膈肌、腓肠肌和心肌氧化性能的影响。方法 :SD大鼠先随机分为 2组 ;肺气肿组经气管内一次性缓慢滴注猪胰弹性蛋白酶 80 0 EU/kg ,正常组一次性气管内滴注等体积 0 9%氯化钠。 4周后分别将 2组大鼠再随机分为静息对照和运动组共 4组 ,即 :正常对照组 (NC ,n=10 ) ,正常运动组 (NE ,n =10 ) ,肺气肿对照组 (EC ,n =12 )和肺气肿运动组 (EE ,n =11)。然后NE组和EE组进行同等强度和时间的电动平板跑步锻炼。在末次运动锻炼结束后 48～ 72小时处死大鼠 ,用组织化学方法染色 ,光密度分析测定膈肌肋部、腓肠肌和心肌纤维琥珀酸脱氢酶 (SDH)和乳酸脱氢酶 (LDH)活性 ,评价相应肌组织的氧化代谢能力。结果 :运动锻炼后NE和EE组心肌和腓肠肌肌纤维氧化性能明显改善 ,SDH活性升高 (P <0 0 5 ) ,但膈肌肌纤维中SDH活性仅在EE组有提高 (P <0 0 5 )。LDH在各组不同肌纤维中均无明显变化。
Objective: To observe the effects of mid-intensity (55% -75% V0 2max) endurance exercise on the diaphragmatic muscle, gastrocnemius and myocardial oxidation in normal and emphysematous rats. Methods: First, SD rats were randomly divided into two groups: emphysema group tracheal intratracheal instillation of porcine pancreatic elastase 80 0 EU / kg, the normal group of a single intratracheal instillation of 0.9% sodium chloride . Four weeks later, the two groups of rats were randomly divided into 4 groups: control group (NC, n = 10), normal exercise group (NE, n = 10), emphysema Control group (EC, n = 12) and emphysema group (EE, n = 11). Then NE and EE groups were given equal treadmill treadmill exercise. The rats were sacrificed at 48 to 72 hours after the end of the last exercise and stained with histochemical method to determine the activity of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) of diaphragmatic muscle, gastrocnemius muscle and cardiac muscle fiber by optical density analysis. Evaluation of the corresponding oxidative metabolism of muscle tissue. RESULTS: After exercise, the oxidative properties of myocardial fibers and gastrocnemius muscles in NE and EE groups were significantly improved and the activity of SDH was increased (P <0.05). However, the activity of SDH in diaphragmatic myofibers was only increased in EE group (P <0.05). LDH in different muscle fibers in each group did not change significantly.