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目的探讨对羟基苯甲醇抗血小板聚集作用及机制。方法以二磷酸腺苷(ADP)、花生四烯酸(AA)为诱导剂,探讨对羟基苯甲醇的体外抗血小板聚集活性;采用下腔静脉结扎致大鼠静脉血栓方法,考察对羟基苯甲醇的体内抗血小板聚集活性;并利用双波长荧光分光光度法测定对羟基苯甲醇对AA诱导的家兔血小板聚集胞浆钙离子浓度的影响。结果对羟基苯甲醇对AA诱导的家兔体外血小板聚集有明显的对抗作用,其半数抑制率(50% inhibitory concentration,IC50)为0.107 g·L-1,对ADP诱导的家兔体外血小板聚集有抑制作用,IC50为0.3 g·L-1;天麻成分对羟基苯甲醇能显著对抗大鼠下腔静脉血栓的形成;对羟基苯甲醇对AA诱导的家兔血小板细胞外钙内流与内钙释放和对照组相比差异均有非常显著性意义。结论天麻成分对羟基苯甲醇在体外、体内均具有显著的抗血小板聚集活性;其作用机制可能是通过抑制外钙内流和内钙释放达到抑制血小板聚集作用。
Objective To investigate the anti-platelet aggregation effect of p-hydroxybenzyl alcohol and its mechanism. Methods The anti-platelet aggregation activity of p-hydroxybenzyl alcohol in vitro was induced by adenosine diphosphate (ADP) and arachidonic acid (AA). Venous thrombosis was induced in rats by inferior vena cava ligation. In vivo anti-platelet aggregation activity. The effect of p-hydroxybenzyl alcohol on AA-induced platelet aggregation cytosolic calcium concentration in rabbits was determined by dual-wavelength fluorescence spectrophotometry. Results Hydroxybenzyl alcohol had a significant antagonistic effect on platelet aggregation induced by AA in vitro. The 50% inhibitory concentration (IC50) was 0.107 g · L-1. The ADP-induced in vitro platelet aggregation in rabbits IC50 was 0.3 g · L-1. Gastrodia component of p-hydroxybenzyl alcohol significantly inhibited the formation of IVC thrombosis in rats; p-hydroxybenzyl alcohol induced platelet extracellular calcium influx and intracellular calcium release in rabbit Compared with the control group, the difference was very significant. Conclusion The constituents of Gastrodia elata Blume have obvious anti-platelet aggregation activity in vitro and in vivo. The possible mechanism is that the inhibition of platelet aggregation may be achieved by inhibiting the influx of calcium and the release of calcium.