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对籼(或粳)型不育系(A)与粳(或籼)型恢复系(R)和粳型广亲和系(WCV)配制的三交F1进行育性鉴定的结果显示,在三交组合籼A//粳R/粳WCV或籼A//粳WCV/粳R、粳A//籼R/粳WCV或粳A//粳WCV/籼R以及籼A/粳WCV//粳R或粳A/粳WCV//籼R的后代中,均可出现一定比例的正常结实株。表明这类植株已同时结合了广亲和基因和恢复基因,并有效地克服了籼粳交不育与细胞质雄性不育。这意味着将广亲和基因导入恢复系,育成广亲和的粳型(或籼型)恢复系,再与籼型(或粳型)不育系配组;或将广亲和基因导入不育系,育成广亲和的籼型(或粳型)不育系,再与粳型(或籼型)恢复系配组,以利用籼粳亚种间杂种优势的途径是可行的。在配组方式上,除籼A/粳R的方式以外,为有利于解决杂种的生育期超亲和米质等问题,粳A/籼R的配组方式也值得重视。文中还对广亲和基因与恢复基因在不同遗传背景下的表达等问题进行了讨论。
The results of fertility identification of three cross F1 cultivars of indica (or Japonica) type sterile line (A) with japonica (or indica) type restorer (R) and japonica wide compatibility (WCV) Japonica A / indica R / Japonica WCV or indica A / / japonica WCV / Japonica R, Japonica A / indica R / Japonica WCV / Japonica A / Japonica WCV / indica R and indica A / Japonica WCV // Japonica R or japonica A / japonica WCV / indica R descendants, there may be a certain percentage of normal colonies. This indicates that these plants have simultaneously combined the broad compatibility and restorer genes and effectively overcome the indica and japonica male sterility and cytoplasmic male sterility. This implies that the broad compatibility gene is introduced into the restorer line to develop a broad compatibility japonica (or indica) restorer line followed by indica (or japonica) male sterility lines; or the introduction of the broadly compatible gene It is feasible to cultivate a broad compatibility indica (or japonica) CMS line with japonica (or indica) restorer lines to take advantage of the heterosis between indica and japonica subspecies. In addition to the method of indica rice A / japonica R, in order to solve the problems such as the super-compatibility and the quality of rice in the growth period of hybrids, it is also worth paying attention to the matching method of japonica A / indica R. The article also discusses the expression of the genes of broad compatibility and restorer in different genetic backgrounds.