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通过计数分泌细胞因子γ－干扰素记忆性Ｔ细胞，观察急性脑梗塞（ＡＣＩ）患者的特异性Ｔ细胞免疫应答。方法采用酶联免疫斑点法（ＥＬＩＳＡ）检测了３２例ＡＣＩ、２４例炎症性神经疾病（ＯＩＮＤ）和２５例其他神经疾病（ＯＮＤ）患者髓鞘素抗原及其多肽应答性Ｔ细胞。结果ＡＣＩ患者外周血髓鞘素碱性蛋白（ＭＢＰ）及其多肽和含脂质蛋白（ＰＬＰ）自身抗原应答性Ｔ细胞数呈显著增高，脑脊液（ＣＳＦ）中ＭＢＰ应答性Ｔ细胞数约为外周血中的１１０倍。结论这种免疫应答可能是继发于急性缺血后的脑组织损伤，且在紧邻靶器官的ＣＳＦ中表现尤为明显，其病理意义还有待进一步探讨。
The specific T cell immune response in patients with acute cerebral infarction (ACI) was observed by counting cytokine interferon-γ-secreting T cells. Methods The myelin antigen and polypeptide-responsive T cells of 32 patients with ACI, 24 patients with inflammatory neuropathies (OIND) and 25 patients with other neurological diseases (OND) were detected by enzyme-linked immunospot assay (ELISA). Results The number of peripheral blood myelin basic protein (MBP) and its peptide and lipopolysaccharide (PLP) autoantigen were significantly increased in ACI patients. The number of MBP responsive T cells in cerebrospinal fluid (CSF) was about peripheral 110 times in blood. Conclusions This immune response may be secondary to brain injury after acute ischemia and is particularly evident in the CSF immediately adjacent to the target organ. The pathological significance needs to be further explored.