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香蕉是我国重要的热带亚热带农作物。由于绝大多数食用香蕉是三倍体,不能通过授粉进行遗传改良,转基因技术就变得尤为重要。建立胚性悬浮细胞系是进行香蕉遗传转化的第1步。但是,建立香蕉胚性悬浮细胞系的成功率极低,直到现在多数实验室还不能自己建立香蕉胚性悬浮细胞系。然而,为什么香蕉胚性悬浮细胞系如此难以建立,在香蕉体细胞向胚性细胞转化的过程中,发生了哪些关键事件,如何提高香蕉胚性愈伤诱导的成功率,关于这些问题,目前还未见到报道。文中对近年来香蕉胚性悬浮细胞系建立方面的工作进行综述,针对香蕉胚性悬浮细胞系建立过程中发生的关键事件进行阐述,对香蕉胚性悬浮细胞系建立困难的原因进行讨论,并提出了若干提高香蕉胚性愈伤诱导成功率的可能途径。
Banana is an important tropical and subtropical crop in China. Since most edible bananas are triploid and can not be genetically modified by pollination, transgenic technology becomes even more important. Establishing embryogenic suspension cell lines is the first step in the genetic transformation of bananas. However, the success of establishing banana embryogenic suspension cell lines is extremely low, and until now most laboratories have not been able to establish banana embryogenic suspension cell lines themselves. However, why banana embryogenic suspension cell lines are so difficult to set up, what are the key events that occur during the transformation of banana somatic cells into embryogenic cells and how to improve the success rate of embryogenic callus induction in banana. For these problems, Did not see the report. In this paper, we summarize the establishment of banana embryogenic suspension cell lines in recent years, and describe the key events in the establishment of banana embryogenic suspension cell lines, and discuss the reasons for the establishment of banana embryogenic suspension cell lines. Some possible ways to improve the success rate of embryogenic callus induction in banana.