论文部分内容阅读
目的:研究阿米洛利衍生物二甲基阿米洛利(DMA)对培养的HL-60细胞增殖和分化的影响。方法:MTT法测定细胞增殖,细胞染色和NBT还原试验测定细胞分化。结果:DMA以浓度依赖方式抑制HL-60细胞增殖,药物作用96小时测得半数抑制浓度(IC_(50))为31.7(95％可信限为6.3-57.1)μmol·L~(-1)。DMA诱导HL-60细胞向粒细胞系分化。加入DMA100μmol·L~(-1)作用3天,细胞分化率从6.5％升至70％。阿米洛利及阿米洛利的另二个衍生物对细胞分化无明显作用。结论:二甲基阿米洛利抑制HL-60细胞增殖和诱导HL-60细胞分化。
Objective: To investigate the effect of amiloride derivative dimethylamilolide (DMA) on the proliferation and differentiation of cultured HL-60 cells. Methods: MTT assay was used to determine cell proliferation, cell staining and NBT reduction assay. Results: The proliferation of HL-60 cells was inhibited by DMA in a concentration-dependent manner. The median inhibitory concentration (IC 50) measured after 96 hours of treatment was 31.7 (95% confidence interval 6.3-57.1) μmol·L -1, . DMA induces HL-60 cells to differentiate into granulocytic lines. After adding 100μmol·L -1 DMA for 3 days, the cell differentiation rate increased from 6.5% to 70%. The other two derivatives of amiloride and amiloride have no significant effect on cell differentiation. Conclusion: Dimethyl amiloride can inhibit the proliferation of HL-60 cells and induce the differentiation of HL-60 cells.