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目的构建表达猪带绦虫六钩蚴期特异性抗原基因TSO45-4B的真核表达质粒pcDNA3.1/TSO45-4B。方法全基因合成TSO45-4B的基因序列,并利用PCR扩增该基因序列,将其插入真核表达载体pcDNA3.1,经酶切、测序鉴定。结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45-4B基因。结论成功构建pcDNA3.1/TSO45-4B重组质粒。
Objective To construct eukaryotic expression plasmid pcDNA3.1 / TSO45-4B expressing the antigen of TSA45-4B of Taenia solium in Taenia. Methods The gene sequence of TSO45-4B was synthesized and the gene sequence was amplified by PCR. The gene was inserted into the eukaryotic expression vector pcDNA3.1 and identified by restriction enzyme digestion and sequencing. Results The enzyme digestion and sequencing showed that the constructed recombinant expression plasmid contained TSO45-4B gene. Conclusion The pcDNA3.1 / TSO45-4B recombinant plasmid was successfully constructed.