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本文报道吡喹酮对体外培养的曼氏血吸虫成虫活动的影响。自感染曼氏血吸虫尾蚴7～10周后的小鼠体内取虫,置于持续流动的含50％血清的培养液内,用多道超声活动记录仪记录虫的活动度。将吡喹酮溶于乙醇中,配成10微克/毫升的浓度,然后再进行稀释,使培养液中的药物浓度范围为0.01～10微克/毫升。在加药前记录虫的活动5小时,作为给药前的对照;加药后继续记录5小时,观察药物对虫体活动的影响。
This article reports the effect of praziquantel on the activity of Schistosoma mansoni adults cultured in vitro. Mice were infected with Schistosoma mansoni cercariae 7 to 10 weeks after taking the body of insects, placed in a continuous flow of 50% serum in the culture medium, recorded with a multi-channel ultrasonic activity recorder insects activity. Praziquantel was dissolved in ethanol to a concentration of 10 μg / ml and then diluted to give a drug concentration in the culture broth of 0.01 to 10 μg / ml. Record the activities of insects 5 hours before dosing as a control before administration; continue recording for 5 hours after dosing, and observe the effect of drugs on the activity of parasites.