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目的:研究二至丸乙酸乙酯提取物(ethyl acetate extractsof Erzhi pill,EAEP)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分成6组:正常对照组、四氯化碳损伤模型组、EAEP高、中、低剂量组(19.8,13.2,6.6 g.kg-1)和联苯双酯组(150 mg.kg-1)。用腹腔注射CCl4致小鼠急性肝损伤模型,测定小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性;测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力;计算肝指数并同时对肝组织进行病理学检查。结果:EAEP具有剂量依赖性地降低CCl4致小鼠肝损伤血清ALT,AST值升高,降低肝匀浆中MDA的含量,增强SOD的活性。通过病理学切片观察,EAEP各剂量组能明显改善肝组织的病理变化。结论:EAEP对四氯化碳造成的小鼠急性肝损伤具有显著的保护作用。
Objective: To study the protective effect of ethyl acetate extract of Erzhi pill (EAEP) on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: Sixty Kunming mice were randomly divided into 6 groups: normal control group, carbon tetrachloride injury model group, EAEP high, medium and low dose groups (19.8, 13.2, 6.6 g.kg-1) and biphenyl Ester group (150 mg.kg-1). The model of acute liver injury induced by CCl4 was induced by intraperitoneal injection. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were measured. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) ) Vitality; calculate the liver index and at the same time, pathological examination of liver tissue. Results: EAEP could reduce ALT and AST in liver of mice induced by CCl4 in a dose-dependent manner, decrease the content of MDA in liver homogenate and increase the activity of SOD. Through the pathological section observation, each EAEP dose group can significantly improve the pathological changes of liver tissue. Conclusion: EAEP has a significant protective effect on CCl4-induced acute liver injury in mice.