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目的建立RP-HPLC法同时测定桂枝茯苓胶囊中没食子酸、白芍苷和芍药苷含量。方法色谱柱:大连中汇达C18柱(4.6 mm×250 mm,5μm),流动相:乙腈-体积分数为0.1%的磷酸溶液,梯度洗脱,流速:1.0 mL.min-1,检测波长:230 nm,柱温:40℃。结果没食子酸、白芍苷和芍药苷的线性分别为0.69~6.87 mg.L-1(r=0.999 6)、0.88~8.80 mg.L-1(r=0.999 6)和2.00~20.00 mg.L-1(r=0.999 5)。平均回收率:没食子酸为99.7%(RSD=1.0%,n=9)、白芍苷为101.5%(RSD=1.5%,n=9)、芍药苷为100.0%(RSD=0.7%,n=9)。结论此方法可为桂枝茯苓胶囊的质量控制提供方法。
OBJECTIVE To establish a RP-HPLC method for simultaneous determination of gallic acid, white peonyone and paeoniflorin in Guizhi Fuling capsule. Methods Column: Dalian Zhonghui C18 column (4.6 mm × 250 mm, 5μm), mobile phase: acetonitrile-0.1% phosphoric acid solution with a gradient elution at a flow rate of 1.0 mL · min- 230 nm, column temperature: 40 ℃. Results The linearities of gallic acid, paeoniflorin and paeoniflorin were 0.69 ~ 6.87 mg.L-1 (r = 0.999 6), 0.88-8.80 mg.L-1 (r = 0.999 6) and 2.00 ~ 20.00 mg.L -1 (r = 0.999 5). The average recovery was 99.7% (RSD = 1.0%, n = 9) for gallic acid, 101.5% for paeoniflorin (RSD = 1.5%, n = 9) and 100.0% for paeoniflorin (RSD = 9). Conclusion This method can provide a method for the quality control of Guizhi Fuling capsule.