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用原位末端标记法（ＴＵＮＥＬ） 、麦格—姬姆萨（ ＭＧＧ） 染色法和免疫组化技术， 观察了经５、１０ 、２０和３０Ｇｙ 不同剂量的γ射线照射后４ ｈ～３６ ｈ， 人Ｊｕｒｋａｔ Ｔ淋巴细胞白血病细胞（简称Ｊｕｒｋａｔ 细胞） 的凋亡及Ｂａｘ和Ｂｃｌ２ 蛋白的表达。结果表明， 不同剂量γ射线照射后， Ｔ淋巴细胞数的减少和凋亡率均显示出较好的量效和时效关系， 证实细胞凋亡是照射后Ｔ淋巴细胞数减少的主要原因之一； Ｂａｘ 和Ｂｃｌ２ 蛋白在上述电离辐射诱发的Ｊｕｒｋａｔ 细胞凋亡的调控中起着重要的作用。
TUNEL, MGG staining and immunohistochemistry were used to observe the effects of different doses of 5, 10, 20 and 30 Gy γ-rays irradiation for 4 h to 36 h, Apoptosis of human Jurkat T lymphocyte leukemia cells (Jurkat cells) and the expression of Bax and Bcl 2 protein. The results showed that the decrease of T lymphocyte number and apoptosis rate showed good dose-effect and time-dependent relationship after irradiation with different doses of γ-rays. It was confirmed that apoptosis was one of the main reasons for the decrease of T lymphocytes after irradiation. Bax and Bcl 2 proteins play an important role in the regulation of apoptosis induced by ionizing radiation in Jurkat cells.