Comparison of Osteogenesis Between Two Kinds of Stem Cells from Goat Combined Calcium Phosphate Ceme

• 作 者: 赵伟 陆家瑜 郝永明 张秀丽 瞿晓辉 华丽 曹春花 邹德荣
• 机 构: Department of Stomatology, the Sixth People's Hospital Affiliated to Shanghai Jiaotong University,Or
• 出 处: Journal of Shanghai Jiaotong University(Science)
• 发表日期: 2011年05期
• 关键词: teeth osteogenesis scaffold alkaline osteopontin cultivation mineralization oste

To explore the possible mechanism of osteogenesis for deciduous teeth stem cells (DTSCs) in vivo/ vitro, stem cells from goat deciduous teeth (SGDs) were firstly isolated, induced and transplanted into immunocompromised mice. The SGDs’s mineralization pattern and osteogenesis were compared with bone marrow messenchymal stem cells (BMMSCs) from goats. SGDs have similar osteogenic differentiation pattern in vitro and bone-like tissue formation mechanism in vivo to BMMSCs; moreover SGDs have stronger alkaline phosphatase (ALP) gene expression and osteopontin (OPN) gene expression levels than BMMSCs; also SGDs can form more bone-like tissues than BMMSCs when cell-scaffold compounds are transplanted into immunocompromised mice. This pre-clinical study in a large-animal model confirms that DTSCs may be an appropriate source of stem cells in repairing bone defects with tissue engineering.

To explore the possible mechanism of osteogenesis for deciduous teeth stem cells (DTSCs) in vivo / vitro, stem cells from goat deciduous teeth (SGDs) were locally isolated, induced and transplanted into immunocompromised mice. The SGDs’ mineralization pattern and osteogenesis were compared with bone moresups have stronger osteogenic differentiation (OPN) gene expression and osteopontin (OPN) gene expression levels than BMMSCs; also SGDs can form more bone-like tissues than BMMSCs when cell-scaffold compounds are transplanted into immunocompromised mice. This pre-clinical study in a large-animal model confirms that DTSCs may be an appropriate source of stem cells in repairing bone defects with tissue engineering.