论文部分内容阅读
目的探讨槐耳清膏抑制人成骨肉瘤Saos-2的作用效果和机制。方法对数期生长人成骨肉瘤Saos-2细胞分为槐耳清膏2、8、16g/L组和空白对照组,分别采用槐耳清膏2、8、16g/L和生理盐水进行处理,分别于处理12、24、36h后,采用MTT法检测各组肿瘤细胞体外增殖情况,采用RT-PCR法检测各组肿瘤细胞趋化因子受体4(chemokine receptor 4,CXCR4)基因表达情况,采用Western blot检测各组肿瘤细胞血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达水平。结果槐耳清膏16g/L组12、24、36h时细胞增殖抑制率分别为(23.30±0.10)%、(43.20±0.53)%、(51.70±0.76)%,8g/L组分别为(7.10±0.08)%、(34.90±0.33)%、(37.40±0.35)%,2g/L组分别为((1.20±0.12)%、(2.97±0.11)%、(5.30±0.17)%,空白对照组分别为(0.30±0.11)%、(1.10±0.09)%、(1.50±0.15)%),除8g/L组24h时与36h时比较差异无统计学意义外,其余各组各时间点两两比较差异均有统计学意义(P<0.05);槐耳清膏16g/L组在12、24、36h细胞CXCR4基因和VEGF蛋白表达水平明显高低于槐耳清膏2、8g/L组和空白对照组(P<0.05),槐耳清膏8g/L组低于2g/L组和空白对照组,2g/L组低于空白对照组(P<0.05);槐耳清膏2、8、16g/L组36h时CXCR4基因和VEGF蛋白表达水平低于12、24h,24h时低于12h时(P<0.05)。结论槐耳清膏在体外能抑制人成骨肉瘤Saos-2细胞的增殖、生长和转移,其作用机制可能同抑制血管生成、调节CXCR4表达有关。
Objective To investigate the effect and mechanism of the Hua-hui cream on human osteosarcoma Saos-2. Methods Saos-2 cells of logarithmic growth osteosarcoma were divided into 2 groups, 8,16 g / L group and blank control group. The cells were treated with 2, 8, and 16 g / L sodium metabisulfite and normal saline respectively , Respectively. The proliferation of tumor cells in each group was detected by MTT assay after 12, 24 and 36 hours. The expression of chemokine receptor 4 (CXCR4) gene in each group was detected by RT-PCR. The protein expression of vascular endothelial growth factor (VEGF) in each group was detected by Western blot. Results The inhibitory rates of cell proliferation were (23.30 ± 0.10)%, (43.20 ± 0.53)% and (51.70 ± 0.76)%, respectively, in the 16g / L group (1.20 ± 0.12)%, (2.97 ± 0.11)% and (5.30 ± 0.17)% respectively in the 2g / L group compared with those in the blank control group (± 0.88%), (34.90 ± 0.33)% and (0.30 ± 0.11)%, (1.10 ± 0.09)%, (1.50 ± 0.15)%, respectively), except for 8g / L group, there was no significant difference at 24h and 36h (P <0.05). The expression levels of CXCR4 and VEGF in the cells of 16g / L group were significantly higher than those of the Hua calmex 2 and 8g / L groups (P <0.05), while those in the Hua-hui Qing-Zhi 8g / L group were lower than those in the 2g / L group and the blank control group, while those in the 2g / L group were lower than those in the blank control group The expression of CXCR4 gene and VEGF protein in the 16g / L group was lower than 12,24h at 36h and lower than 12h at 24h (P <0.05). CONCLUSION: IPT can inhibit the proliferation, growth and metastasis of human osteosarcoma cell line Saos-2 in vitro. The mechanism may be related to the inhibition of angiogenesis and the regulation of CXCR4 expression.