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目的 从人羊水中提取和纯化肺表面活性物质相关蛋白Ａ（ＳＰ－Ａ）。方法 收集１１例产妇的羊水共４０００ｍｌ，采用 萃取和透析的方法提纯人羊水中ＳＰ－Ａ并测定其生物学活性。结果 获得 ＳＰ－Ａ １２ ｍｇ（共 ２０ ｍｌ）；ＳＤＳ－ＰＡＧＥ显示，提纯 后的ＳＰ－Ａ的相对分子质量为 ３１ ０００，只显示１条条带；Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ也显示１条特异性反应条带；将其加入人工合 成的磷脂中表现为表面活性明显增加，γｍｉｎ由７．７±０．３降至１．５±０．２２。结论 用萃取和透析方法能较好地提纯ＳＰ－Ａ．且纯 度较高，并能较好地保持其生物活性。
Objective To extract and purify pulmonary surfactant associated protein A (SP-A) from human amniotic fluid. Methods A total of 4000 ml of amniotic fluid collected from 11 mothers were collected. The SP-A of human amniotic fluid was purified by extraction and dialysis, and its biological activity was determined. Results The SP-A 12 mg (20 ml total) was obtained. SDS-PAGE showed that the relative molecular mass of the purified SP-A was 31 000, showing only one band. Western blotting also showed one specific band The surface activity of the synthesized phospholipids increased obviously from 7.7 ± 0.3 to 1.5 ± 0.22. Conclusion The extraction and dialysis methods can better purify SP-A. And high purity, and can better maintain its biological activity.