论文部分内容阅读
为探讨ＰＭＬ（Ｐｒｏｍｙｅｌｏｃｙｔｉｃｌｅａｋｅｍｉａ）生长抑制因子对人前列腺癌细胞的作用，采用ｌｉｐｏｆｅｃｔａｍｉｎｅ介导基因转染技术将ＰＭＬ基因和Ｇ４１８抗性基因联合导入体外培养的人前列腺癌细胞，经Ｇ４１８筛选、Ｗｅｓｔｅｒｎｂｌｏｔ杂交检测出ＰＭＬ蛋白过表达细胞克隆，经体外生长抑制试验和裸鼠体内致瘤能力试验，观察ＰＭＬ过表达对前列腺癌细胞体外生长和裸鼠体内致瘤能力的影响。结果证实ＰＭＬ基因被成功地导入前列腺癌细胞中并得到高效表达。过表达ＰＭＬ蛋白的细胞体外生长能力和裸鼠体内致瘤能力明显低于对照细胞。提示ＰＭＬ生长抑制因子过表达能够有效地抑制前列腺癌细胞的体外生长和裸鼠体内的致瘤能力。
To investigate the effect of growth inhibitory factor (PML) on human prostate cancer cells, lipofec-tamine-mediated gene transfection was used to introduce PML gene and G418 resistance gene into cultured human prostate cancer cells. After G418 selection, Western blot Hybridization detected PML protein overexpression cell clones, in vitro growth inhibition test and nude mice in vivo tumorigenicity test to observe the effect of PML overexpression on prostate cancer cell growth in vitro and nude mice in vivo tumorigenic ability. The results confirmed that the PML gene was successfully introduced into prostate cancer cells and was highly expressed. The in vitro growth ability of PML-overexpressing cells was significantly lower than that of control cells in nude mice. Tip PML growth factor over-expression can effectively inhibit the growth of prostate cancer cells in vitro and in vivo tumorigenic capacity in nude mice.