论文部分内容阅读
在茶树抗性育种中,常常需要鉴定茶树抗寒性的强弱。田间自然越冬调查是测定茶树抗寒性强弱的常规方法,然而,田间调查法受制于外界温度的波动,干扰较大,在实际应用中难以获得满意结果。“TTC”分析法是一种广泛用于测定细胞活力和抗寒性强弱的技术,Purcell等人(1963)曾用TTC法进行柑桔、苹果等果树的田间冻害测定,Steponkus等人(1967)将TTC法加以改进,用于木本植物冰冻处理后组织活力的定量分析,桔尚等人(1976)也曾将该法用于测定茶树根系活力,但在茶树抗寒性鉴定中应用TTC法
In tea resistant breeding, it is often necessary to identify the strength of tea cold hardiness. However, the field survey method is subject to fluctuations in the external temperature and has large interference, making it difficult to obtain satisfactory results in practical applications. The “TTC” assay is a widely used technique for the determination of cell viability and cold tolerance. Purcell et al. (1963) used the TTC method to determine field frost damage in citrus, apple and other fruit trees. Steponkus et al. (1967 ) TTC method was improved for the quantitative analysis of tissue vitality after freezing treatment of woody plants, Kishang et al (1976) have also used this method for the determination of tea root activity, but the identification of cold hardiness in tea applications TTC law