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目的:研究趋化因子受体CXCR4在结直肠癌组织中的表达,探讨其与淋巴结、肝转移及预后的关系。方法:应用免疫组化方法(SP法)检测20例正常结直肠黏膜组织、64例结直肠癌组织、34例区域淋巴结转移癌组织以及18例肝转移组织CXCR4表达情况。同时应用逆转录聚合酶链反应(RT-PCR)检测其mRNA在4株大肠癌细胞株中的表达。结果:正常结直肠黏膜、结直肠癌、区域淋巴结转移癌以及肝转移组织中CXCR4阳性率分别为15.0%、51.6%、72.2%和73.5%;转移组织阳性表达率明显高于原发肿瘤。CX-CR4 mRNA高表达于2株人大肠癌细胞株。结直肠癌组织CXCR4阳性表达率与Duke临床分期、淋巴结转移和肝转移密切相关,P<0.05,而与患者年龄、性别、肿瘤所在部位及病理分化程度无关。CXCR4阳性表达组3年生存率明显低于阴性表达组,P<0.05。结论:CXCR4阳性表达与结肠直肠癌的淋巴结、肝转移有关,有助于预后判断。
Objective: To study the expression of chemokine receptor CXCR4 in colorectal cancer and its relationship with lymph node, liver metastasis and prognosis. Methods: The expression of CXCR4 in 20 cases of normal colorectal mucosa, 64 cases of colorectal cancer, 34 cases of regional lymph node metastasis and 18 cases of liver metastasis were detected by immunohistochemistry (SP method). At the same time, reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression in the four colorectal cancer cell lines. Results: The positive rates of CXCR4 in normal colorectal mucosa, colorectal cancer, regional lymph node metastasis and liver metastasis were 15.0%, 51.6%, 72.2% and 73.5%, respectively. The positive rate of CXCR4 in metastatic tissues was significantly higher than that in primary tumors. CX-CR4 mRNA is highly expressed in two human colorectal cancer cell lines. The positive rate of CXCR4 expression in colorectal cancer was closely related to Duke clinical stage, lymph node metastasis and liver metastasis (P <0.05), but not with age, sex, tumor location and pathological grade. The 3-year survival rate of CXCR4 positive group was significantly lower than that of negative group (P <0.05). Conclusion: The positive expression of CXCR4 is associated with lymph node and liver metastasis in colorectal cancer, which is helpful to prognosis.