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采用Ｓｐｅｒｍｏｓｐｈｅｒｅｍｏｄｅｌｓ测定５种培养基铁浓度下两株非共生固氮菌（巴西固氮螺菌ＮＯ４０和多粘芽孢杆菌）及两者的混合菌分别与两个小麦基因型（Ｇｉｚａ１６７和Ｓａｋｈａ８）的联合固氮效率，发现培养基的铁浓度对联合固氮效率具有显著的影响．若不考虑菌株间和小麦基因型间的差异，培养基铁浓度在２．５８３～１４．７１５ｍｇ／Ｌ时的联合固氮效率显著高于铁浓度为４２．８８３～１６６．３１ｍｇ／Ｌ；联合固氮效率在小麦基因型间未表现出显著差异，但在不同菌株之间差异极显著．表现为：巴西固氮螺菌＞混合菌＞多粘芽孢杆菌；联合固氮效率在培养基铁浓度与不同菌株之间存在明显的交互作用，巴西固氮螺菌对铁浓度的适应范围比多粘芽孢杆菌及混合菌广，在固氮高峰期巴西固氮螺菌与小麦基因型Ｇｉｚａ１６７组合的联合固氮效率在５种供试铁浓度下均达较高水平而未表现出显著的差异，巴西固氮螺菌与Ｓａｋｈａ８组合的联合固氮效率在铁浓度为２．５８３～４２．８８３ｍｇ／Ｌ时最高．说明巴西固氮螺菌ＮＯ４０对培养基中供铁水平的适应范围较广，因而具有良好的应用前景．
Spermospheremodels were used to determine the combined nitrogen fixation efficiency of two non-symbiotic Azotobacter species (Azotobacterium sp. NO40 and Bacillus polymyxa) and their two mixed genotypes with two wheat genotypes (Giza167 and Sakha8) , It was found that the iron concentration of the medium had a significant effect on the combined nitrogen fixation efficiency. Regardless of the difference between strains and wheat genotypes, the combined nitrogen fixation efficiency of medium iron concentration 2.583-14.715mg / L was significantly higher than that of iron concentration 42.883-166.31mg / L. Combined nitrogen fixation Efficiency did not show significant differences among wheat genotypes, but significant differences were observed among different strains. The results showed as follows: Azospirillum brasilense> mixed bacterium> Bacillus polymyxa; the combined nitrogen fixation efficiency had obvious interaction with iron concentration in culture medium and different strains, And mixed bacteria wide, at the peak of nitrogen fixation, the combined nitrogen fixation efficiency of Azospirilum brasiliensis and wheat genotype Giza167 reached a high level under the five tested iron concentrations without significant difference, and Azospirillum brazilei and Sakha8 The combination of nitrogen fixation efficiency at the iron concentration of 2.583 ~ 42.883mg / L when the highest. These results indicated that Azotobacterium sp. NO40 had a wide range of adaptability to iron supply in culture medium and had a good application prospect.