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大麦网斑病是大麦(Hordeum vulgare)主要病害之一,由网斑病菌(Pyrenophora teres)引起。近年来网斑病在我国大麦产区发生并流行,网斑病之所以在我国各大麦产区发生并流行,主要是由于生产中缺乏抗网斑病品种。为分析大麦种质材料遗传多样性,筛选与大麦抗网斑病性状相关联的SSR标记,本研究利用70对品种间表现多态性的SSR标记对89个大麦品种(系)进行分析。结果显示,70个SSR标记共检测出302个等位变异,平均4.31个,变幅为2~8个;等位基因频率为0.141 2~0.916 7;基因多样性和遗传相似系数的变化范围分别是0.103 9~0.894 4和0.520~0.873;多态性信息含量(polymorphic information content,PIC)为0.098 5~0.884 6,平均为0.537。群体遗传结构分析表明,供试大麦亲本材料可分为2个亚群,群体遗传相似系数变幅为0.635~0.895。品种美41/I和美43/I的遗传相似系数最高,为0.895。根据一般线性模型(general linear model,GLM)分析,发现5个与大麦抗网斑病相关的标记,解释率在7.2%~22.4%之间,标记Bmac29和Bmag0613关联达到极显著水平(P<0.01),对表型变异的解释率为分别为10.7%和22.4%。本研究为大麦网斑病抗病育种提供了一定理论依据。
Barley net blotch is one of the major diseases of barley (Hordeum vulgare), caused by Pyrenophora teres. In recent years, the occurrence and prevalence of net blotch in barley producing areas in China. The reason why net blotch occurs and prevails in all major barley producing areas in our country is mainly due to the lack of varieties resistant to net blotch in production. In order to analyze the genetic diversity of barley germplasm materials and screen SSR markers associated with the resistance of barley to net blotch, 89 barley cultivars (lines) were analyzed using SSR markers of 70 pairs of polymorphisms among varieties. The results showed that 70 SSR markers detected 302 alleles with an average of 4.31 loci, ranging from 2 to 8 loci; the allele frequencies ranged from 0.141 2 to 0.916 7. The range of genetic diversity and genetic similarity coefficient Were 0.103 9 ~ 0.894 4 and 0.520 ~ 0.873. The polymorphic information content (PIC) was 0.098 5 ~ 0.884 6 with an average of 0.537. The analysis of population genetic structure showed that the parents of the tested barley could be divided into two subgroups, and the genetic similarity coefficient of the population varied from 0.635 to 0.895. The genetic similarity coefficient of varieties 41 / I and 43 / I was the highest, 0.895. According to the general linear model (GLM) analysis, five markers related to cytoplasm reticuloplasma were found, the interpretation rates were between 7.2% and 22.4%, and the correlation between markers Bmac29 and Bmag0613 was extremely significant (P <0.01 ), Explaining the phenotypic variation at 10.7% and 22.4%, respectively. This study provides a theoretical basis for disease resistance breeding of barley net blotch.