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目的观察环磷腺苷葡氨(MCA)和氨茶碱联用对小鼠急性缺氧性脑损伤(HBI)的干预作用,初步探讨其可能的神经保护机制。方法90只小鼠分为正常对照组、HBI组、氨茶碱组、MCA组、两药合用组。缺氧后72h测各组脑组织含水量、超氧化物歧化酶活性(SOD)及氧化代谢产物丙二醛(MDA)含量;HE染色观察各组海马区结构及神经细胞的调亡情况。结果与HBI模型组相比,药物干预组脑组织含水量、MDA下降,SOD升高,病理变化减轻,细胞调亡减少,病理变化尤以两药合用组改善最显著。结论两药联用可有效抑制缺氧后脑组织内氧自由基生成,减轻脑水肿及神经细胞的调亡。
Objective To investigate the intervention of cyclic adenosine monophosphate (MCA) and aminophylline on acute hypoxic brain injury (HBI) in mice and to explore its possible neuroprotective mechanism. Methods 90 mice were divided into normal control group, HBI group, aminophylline group, MCA group and two drugs combined group. At 72 hours after hypoxia, the water content, superoxide dismutase (SOD) and malondialdehyde (MDA) contents were measured in each group. The structure of hippocampus and the apoptosis of neurons in each group were observed by HE staining. Results Compared with the HBI model group, the water content, MDA, SOD in the intervention group decreased, the pathological changes were alleviated and the apoptosis rate decreased. The pathological changes especially in the combination of the two drugs were the most significant. Conclusions The combination of the two drugs can effectively inhibit the generation of oxygen free radicals in the brain tissue after hypoxia, and reduce the brain edema and the apoptosis of nerve cells.