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目的:探讨自动顶空气相色谱法测定血液中的乙醇。方法:取一定量的血液样品,加入叔丁醇作为内标,用顶空自动进样气相色谱火焰离子化检测器进行检测,以保留时间定性,内标法、峰面积比进行定量分析。结果:乙醇浓度在0.10 mg/ml～3.00 mg/ml范围内有好的线性(r=0.9999),检出限为0.01 mg/ml,样品的加标回收率为97.8%～104.3%,RSD<2.0%。结论:所建立的方法简便、快速、准确,可用于血液中乙醇含量的检验。
Objective: To investigate the determination of ethanol in blood by automatic headspace gas chromatography. Methods: A certain amount of blood samples were taken, and t-butanol was added as an internal standard. The samples were detected by headspace automatic gas chromatography with flame ionization detector. The retention time, internal standard method and peak area ratio were quantitatively analyzed. Results: The linearity (r = 0.9999) of alcohol concentration ranged from 0.10 mg / ml to 3.00 mg / ml with the detection limit of 0.01 mg / ml. The recoveries of spiked samples ranged from 97.8% to 104.3% 2.0%. Conclusion: The established method is simple, rapid and accurate, which can be used to test the content of ethanol in the blood.