论文部分内容阅读
目的研究蛋白磷酸酶2A癌性抑制因子(cancerous inhibitor of protein phosphatase 2A,CIP2A)在膀胱尿路上皮癌组织中的表达及其与临床病理特征的关系,探讨其成为膀胱尿路上皮癌预后指标的可行性。方法应用RT-PCR和Western blot检测CIP2A mRNA和蛋白在25例膀胱尿路上皮癌和对应癌旁组织中的表达情况;应用组织芯片技术和免疫组织化学方法,检测CIP2A在117例膀胱尿路上皮癌和30例癌旁组织中的表达情况,分析CIP2A与膀胱尿路上皮癌患者临床病理特征及预后之间的关系。结果CIP2A mRNA和蛋白在25例配对膀胱尿路上皮癌组织中的表达水平明显高于癌旁组织。免疫组织化学检测发现,膀胱尿路上皮癌组织中CIP2A蛋白的阳性表达率为76.9%(90/117),明显高于癌旁组织的6.7%(2/30),差异有统计学意义(P<0.001)。CIP2A表达与肿瘤病理分级(P<0.001)、临床分期(P<0.001)、肿瘤大小(P=0.002)和淋巴结转移(P=0.046)有关,但与年龄、性别及肿瘤数目无关(P>0.05)。Kaplan-Meier单因素分析显示,CIP2A蛋白高表达是总体生存率和无复发生存率的影响因素(P<0.001)。Cox多因素风险比例模型显示,与总生存率相关的独立预后因素为临床分期、肿瘤病理分级和CIP2A表达,与无复发生存率相关的独立预后因素亦为临床分期、肿瘤病理分级和CIP2A表达。结论CIP2A蛋白在膀胱尿路上皮癌组织中高表达,可能与膀胱尿路上皮癌的进展有关,其表达状态是膀胱尿路上皮癌患者独立预后因素。
Objective To investigate the expression of CIP2A in bladder urothelial carcinoma and its relationship with clinicopathological features and to explore the possible role of CIP2A in the prognosis of bladder urothelial carcinoma feasibility. Methods RT-PCR and Western blot were used to detect the expression of CIP2A mRNA and protein in 25 cases of bladder urothelial carcinoma and corresponding paracancerous tissues. Tissue microarray and immunohistochemistry were used to detect the expression of CIP2A in 117 cases of bladder urothelial Cancer and 30 cases of paracancerous tissues. The relationship between CIP2A and clinicopathological characteristics and prognosis of bladder urothelial carcinoma was analyzed. Results The expression of CIP2A mRNA and protein in 25 paired urothelial carcinoma tissues was significantly higher than that in paracancerous tissues. Immunohistochemistry showed that the positive expression rate of CIP2A protein in bladder urothelial carcinoma tissues was 76.9% (90/117), which was significantly higher than that in paracancerous tissues (2/30) (P <0.05), the difference was statistically significant (P <0.001). CIP2A expression was correlated with tumor pathological grade (P <0.001), clinical stage (P <0.001), tumor size (P = 0.002) and lymph node metastasis (P = 0.046), but not with age, gender and tumor number ). Kaplan-Meier univariate analysis showed that CIP2A protein overexpression was the influencing factor of overall survival and recurrence-free survival (P <0.001). The Cox multivariate risk proportional model showed that the independent prognostic factors associated with overall survival were clinical stage, tumor grade and CIP2A expression. The independent prognostic factors associated with recurrence-free survival were also clinical stage, tumor grade and CIP2A expression. Conclusion The high expression of CIP2A protein in bladder urothelial carcinoma may be related to the progress of bladder urothelial carcinoma and its expression is an independent prognostic factor in patients with urothelial carcinoma.