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应用植物组织培养技术，探讨利用脱毒种蒜茎尖周围鳞茎组织进行扩繁的新途径。结果表明：以ＭＳ为基本培养基，诱导鳞茎愈伤组织形成及分化幼芽的最佳激素浓度组合为２，４—Ｄ０．１ｍｇ／Ｌ＋ＢＡ０．３ｍｇ／Ｌ，切割幼芽接种于ＮＡＡ；ＢＡ不同激素浓度处理的培养基中，促使幼苗株高，叶片生长的最佳激素浓度组合为ＮＡＡ０．０５ｍｇ／＋ＢＡ０．５ｍｇ／Ｌ。因此，切取脱毒种蒜茎尖周围０．２—０．３ｍｍ鳞茎组织，经愈伤组织诱导，幼芽分化，幼苗生长三个阶段１１０天培养，可获得４株试管成苗，并且经幼苗根尖染色体压片，镜检，未发现有任何染色体变异。
Application of plant tissue culture technology to explore the use of detoxification garlic bulbs around the bulbs tip propagation of new ways. The results showed that the best hormone concentration combination of 2,4-D0.1mg / L + BA0.3mg / L with MS as basic medium to induce bulb callus formation and differentiation of young shoots was inoculated on NAA; Hormone concentration in the medium, to promote seedling height, the best hormone combination of leaf growth for NAA0.05mg / + BA0.5mg / L. Therefore, the detoxification of garlic shoots around the tip of 0.2-0.3mm bulb tissue, callus induction, differentiation of young shoots, seedlings growing 110 days three stages of culture can be obtained 4 tube seedlings, and the seedling Root tip chromosomes, microscopic examination, did not find any chromosomal variation.