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目的:研究启膈散、沙参麦冬汤和通幽汤对人食管癌EC9706细胞增殖影响,并从Caspase-3介导的细胞凋亡信号转导通路探讨三方作用机制。方法:体外培养食管鳞癌细胞株EC9706细胞,分别加入启膈散、沙参麦冬汤和通幽汤提取液培养处理细胞,通过MTT染色观察细胞增殖变化,通过Western blot法检测Caspase-3介导的细胞凋亡信号转导通路蛋白表达。结果:启膈散、沙参麦冬汤和通幽汤的IC50值分别是1462、1232、2875μg/m L,均可促进Caspase-3蛋白表达而诱导细胞凋亡,而Cyt.C蛋白表达较弱;启膈散和沙参麦冬汤对FADD、Fas、TNFR1和DR5蛋白表达有不同程度地影响。结论:三方可通过激活Caspase-3诱导细胞凋亡,其中启膈散主要通过非依赖FADD死亡受体途径,沙参麦冬汤主要通过依赖FADD死亡受体途径,通幽汤可能通过其他凋亡蛋白参与的上游信号途径而激活Caspase-3致细胞凋亡。
Objective: To investigate the effects of Qige San, Sha Shen Maidong Tang and Tongyou Tang on the proliferation of human esophageal carcinoma EC9706 cells and to explore the mechanism of action of the three mechanisms by Caspase-3-mediated signal transduction pathway. Methods: The esophageal squamous cell carcinoma cell line EC9706 was cultured in vitro. The cells were cultured and treated with Qige San, Sha Shen Mai Dong Tang and Tongyou Decoction. The proliferation of esophageal squamous cell carcinoma cell line EC9706 was observed by MTT staining. The expression of Caspase-3 Apoptosis Signal Transduction Pathway Protein Expression. Results: The IC50 values of Qige San, Sha Shen Maidong Tang and Tongyou Decoction were 1462, 1232 and 2875 μg / m L, respectively. Both of them could promote the expression of Caspase-3 and induce the apoptosis, while the expression of Cyt.C was Weak; Qige San and Sha Shen Mai Maotang Decoction have different effects on FADD, Fas, TNFR1 and DR5 protein expression. CONCLUSIONS: Three parties can induce apoptosis by activating Caspase-3, of which QG can mainly pass through the pathway of death-independent FADD receptor, SARS via FADD-dependent death receptor pathway, Tongyou Decoction may through other apoptosis Protein involved in the upstream signaling pathway to activate Caspase-3 induced apoptosis.