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HBcAg可从人肝中提取,亦可用基因工程方法生产。我们应用DNA体外重组技术从Adw_2 HBV DNA上用酶切下带有HBcAg基因的DNA片段,与几种pBR322衍生的质粒载体构成重组质粒,转入大肠杆菌获得能合成HBcAg的转化菌株。实验证明这种大肠杆菌合成的HBcAg具有与从人肝提取的HBcAg相同的血清学和免疫学特异性。我们对转化株的营养基成份、培养条件、基因工程后处理进行了综合研究,明显提高了HBcAg产量。利用某些絮凝剂能沉
HBcAg can be extracted from human liver, can also be genetically engineered methods of production. We use DNA in vitro recombination technology to digest the DNA fragment carrying HBcAg gene from Adw_2 HBV DNA and make recombinant plasmid with several plasmid vectors derived from pBR322 and transform into E.coli to obtain transformed strains capable of synthesizing HBcAg. It has been experimentally demonstrated that this E. coli-synthesized HBcAg has the same serological and immunological specificity as HBcAg extracted from human liver. We conducted a comprehensive study on the nutrient composition, culture conditions and genetic engineering of the transformants, and significantly increased the yield of HBcAg. Use some flocculants can sink