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目的探讨吴茱萸碱(EVO)对人肝癌Hep G2细胞增殖的影响及作用机制。方法以人肝癌细胞株(Hep G2细胞)为受试细胞,实验设对照组、吴茱萸碱5.0、12.5、25.0、50.0、100.0μmol/L组,采用噻唑蓝法检测Hep G2细胞增殖抑制率,划痕实验检测Hep G2细胞伤痕愈合率,Western blot检测Hep G2细胞基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白。结果吴茱萸碱对Hep G2细胞增殖抑制率明显高于对照组,呈现时间-剂量依赖关系(P<0.05);与对照组比较,吴茱萸碱5.0、25.0、50.0μmol/L组Hep G2细胞24、48 h伤痕愈合率[分别为(23.31±1.03)%、(10.37±1.06)%、(7.92±1.40)%和(13.86±5.57)%、(1.78±3.08)%、0%]明显下降,差异有统计学意义(P<0.05);与对照组比较,吴茱萸碱5.0、25.0、50.0μmol/L组Hep G2细胞24 h MMP-9、PCNA蛋白表达量[分别为(0.53±0.020)、(0.35±0.020)、(0.21±0.015)与(0.76±0.032)、(0.66±0.01)、(0.59±0.015)]降低,差异有统计学意义(P<0.05)。结论吴茱萸碱可明显抑制Hep G2细胞增殖及远处迁徙,其机制可能与吴茱萸碱下调MMP-9、PCNA蛋白有关。
Objective To investigate the effect of evodiamine (EVO) on the proliferation of Hep G2 cells and its mechanism. Methods Hep G2 cells were used as experimental cells. The control group, evodiamine 5.0,12.5,25.0,50.0,100.0μmol / L group were used to test the inhibition rate of proliferation of Hep G2 cells. The wound healing rate of Hep G2 cells was detected by hematoxylin-eosin staining and the protein expression of matrix metalloproteinase-9 (MMP-9) and proliferating cell nuclear antigen (PCNA) in Hep G2 cells was detected by Western blot. Results Evodiamine significantly inhibited the proliferation of Hep G2 cells in a dose-dependent manner (P <0.05). Compared with the control group, evodiamine 5.0, 25.0, and 50.0 μmol / L Hep G2 cells 24, 48 h (23.31 ± 1.03)%, (10.37 ± 1.06)%, (7.92 ± 1.40)% and (13.86 ± 5.57)%, (1.78 ± 3.08)%, 0% (P <0.05). Compared with the control group, the protein expression of MMP-9 and PCNA in Hep G2 cells in evodiamine 5.0, 25.0 and 50.0 μmol / L groups for 24 h was significantly higher than that in control group [(0.53 ± 0.020) and 0.020), (0.21 ± 0.015) and (0.76 ± 0.032), (0.66 ± 0.01) and (0.59 ± 0.015), respectively. The difference was statistically significant (P <0.05). Conclusion Evodiamine significantly inhibits the proliferation and distant migration of Hep G2 cells, which may be related to the downregulation of MMP-9 and PCNA protein by evodiamine.