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Ｓｊ６５蛋白为来源于日本血吸虫虫卵的循环抗原，该蛋白免疫原性较弱，能与一种分子量为６８ＫＤ的糖蛋白（Ｓｊ６５结合蛋白）专一结合。利用酶标记Ｓｊ６５结合蛋白，建立Ｄｏｔ－ＥＬＩＳＡ检测感染血清中的Ｓｊ６５。用该法测定急、慢性血吸虫病人血清，阳性率分别为１００％及８１．３％。正常人、兔、鼠、肝炎病人血清及蛔、钩虫病人血清均未见阳性反应。在感染兔血清及并殖吸虫病人血清中可检出Ｓｊ６５，而在感染小鼠血清及并殖吸虫感染犬血清中却未检出。感染兔经吡喹酮治疗５周后，９０％转为阴性。慢性血吸虫病人经吡喹酮治疗１年后，６０％转为阴性。结果表明，感染血清中Ｓｊ６５的生化检测似能反映感染程度及考核治疗效果，同时也提示，血清中Ｓｊ６５的水平可因宿主的不同而不同。
The Sj65 protein is a circulating antigen derived from Schistosoma japonicum eggs and has weak immunogenicity and can specifically bind to a glycoprotein (Sj65-binding protein) with a molecular weight of 68KD. Sj65 binding protein was detected by enzyme-linked immunosorbent assay (ELISA). Dot-ELISA was used to detect Sj65 in sera. The method for the determination of acute and chronic schistosomiasis serum, the positive rates were 100% and 81.3%. Normal people, rabbits, mice, hepatitis patients serum and roundworm, hookworm serum no positive reaction. Sj65 was detected in the sera from infected rabbits and paragonimiasis patients, but not from the sera from infected mice or from paragonimus infected dogs. Infected rabbit after praziquantel treatment for 5 weeks, 90% turned negative. Chronic schistosomiasis patients after praziquantel treatment for 1 year, 60% turned negative. The results showed that the biochemical detection of Sj65 in serum could reflect the degree of infection and evaluate the therapeutic effect. It also suggested that the level of Sj65 in serum may vary from host to host.