In vitro and in vivo evaluation of implantable bacterial-killing coatings based on host defense pept

• 作 者: Yuxin Qian Shuai Deng Xue Wu Yunrui She Runhui Liu Haodong Lin
• 机 构: State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shang
• 出 处: 材料科学技术（英文版）
• 发表日期: 2021年32期
• 关键词: Contact-killing Antimicrobial surface HDPs HDP mimic Covalent binding

Contact-killing antimicrobial coatings based on host defense peptides (HDPs) and their synthetic mimics have shown potential as powerful tools to combat implant-associated infections.Covalent modification of the antimicrobial surface has been utilized to prevent early-stage microbial infections owing to the less drug-leaching possibility that is beneficial to human health and the natural environment.Although con-siderable progress has been achieved in preparing contact-killing antimicrobial surfaces,discussions fo-cusing on the in vitro and in vivo evaluations of these surfaces are limited.In this review,we summarized the established in vitro methods to simulate the practical interaction of microbes with the surrounding biological environment and the reported in vivo studies at different implant sites.We suggested that the in vivo specific site infection model is essential to gain a comprehensive understanding of these an-timicrobial coatings in the preclinical stage,which can be established based on investigations performed using various in vitro assays and conventional non-specific site infection models.Overall,these precedent studies focusing on bacterial contact-killing coatings modified with HDPs and HDP mimics can be con-sidered as critical to assess the surface antibacterial ability and to guide the future developments and applications of antimicrobial surfaces.