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Objective:Diarrhea is a leading cause of morbidity and mortality among children in developing countries.The bacterial pathogen most commonly associated with childhood diarrhea is Escherichia coli.A one-year prospective study was carried out in Sanandaj to determine the prevalence and roles of the different E.coli pathotypes in children less than five years of age with acute diarrhea.Methods:Rectal swab were collected prospectively from children with acute diarrhea and transported to the Department of Microbiology,School of Medicine, KUMS,Sanandaj during 2008.The study was approved by the institutional ethics committee.Results:During this study period,rectal swabs were investigated from a total of 466 children 1 to 144 months of age(mean, 29.97 months±S.D) with diarrhea.Among the children,191(41%,191/466) were girls,and 275 (59%,275/466) were boys.The age-specific incidence rates of acute diarrhea among children 13-24 and 1 - 12 months of age were 37.37%(37/99) and 26.26%(26/99),respectively,during the study period.A total of 99 strains of E.coli were detected.EPEC 59(59.59%) and EIEC 22(22.22%),were the most commonly found Escherichia coli strains detected in stools from children.Disk diffusion testing showed E.coli strains resistance to tetracycline(89.89%),chloramphenicol(88.88%),Ampicillin(79.79%),Amoxicillin (75.75%) and Ceficime(75.75%).Among risk factors like age,sex,haemoglubin,fathers and mothers education,food and weight of children only mother’s education was significant(P =0.018).Conclusion: In most of the clinical laboratories in Iran,E.coli does not considered as an etiologic agent responsible for diarrhea. Results in this study revealed that E.coli should be considered as an etiologic agent causing acute diarrhea among children.We therefore,recommend the routine isolation and identification of E.coli strains in all the clinical laboratories in Sanandaj.Guidelines for appropriate use of antibiotics in Sanandaj need updating.
Objective: Diarrhea is a leading cause of morbidity and mortality among children in developing countries. The bacterial pathogen most commonly associated with childhood diarrhea is Escherichia coli. A one-year prospective study was carried out in Sanandaj to determine the prevalence and roles of the different E.coli pathotypes in children less than five years of age with acute diarrhea. Methods: Rectal swab were collected prospectively from children with acute diarrhea and transported to the Department of Microbiology, School of Medicine, KUMS, Sanandaj during 2008. The study was approved by the institutional ethics committee. Results: During this study period, rectal swabs were investigated from a total of 466 children 1 to 144 months of age (mean, 29.97 months ± SD) with diarrhea. Among the children, 191 (41%, 191 / 466) were girls, and 275 (59%, 275/466) were boys. The age-specific incidence rates of acute diarrhea among children 13-24 and 1-12 months of age were 37.37% (37/99) and 26.26 % (26/99), respec A total of 99 strains of E. coli were detected. EPEC 59 (59.59%) and EIEC 22 (22.22%), were the most commonly found Escherichia coli strains detected in stools from children. Disk diffusion testing showed the risk of resistance to tetracycline (89.89%), chloramphenicol (88.88%), Ampicillin (79.79%), Amoxicillin (75.75%) and Ceficime (75.75%). Among risk factors like age, sex, haemoglubin, fathers and mothers education, food and weight of children only mother’s education was significant (P = 0.018) .Conclusion: In most of the clinical laboratories in Iran, E.coli doesnt consider as an etiologic agent responsible for diarrhea. Results in this study revealed that E . coli should be considered as an etiologic agent causing acute diarrhea among children. We therefore, recommend the routine isolation and identification of E. coli strains in all the clinical laboratories in Sanandaj.Guidelines for appropriate use of antibiotics in Sanandaj need updating.