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AIM:To clone,identify and study new NS5ATP2 gene andits spliced variant transactivated by hepatitis C virus non-structural protein 5A.METHODS:On the basis of subtractive cDNA library of genestransactivated by NS5A protein of hepatitis C virus,the codingsequence of new gene and its spliced variant were obtainedby bioinformatics method.Polymerase chain reaction(PCR)was conducted to amplify NS5ATP2 gene.RESULTS:The coding sequence of a new gene and itsspliced variant were cloned and identified successfully.CONCLUSION:A new gene has been recognized as thenew target transactivated by HCV NS5A protein.These resultsbrought some new clues for studying the biological functionsof new genes and pathogenesis of the viral proteins.
AIM: To clone, identify and study new NS5ATP2 gene andits spliced variant transactivated by hepatitis C virus non-structural protein 5 A. METHODS: On the basis of subtractive cDNA library of genestransactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and Its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene .RESULTS: The coding sequence of a new gene and itsspliced variant were cloned and identified successfully. CONCLUSION: A new gene has been recognized as the new target transactivated by HCV NS5A protein. These resultsbrought some new clues for studying the biological functionsof new genes and pathogenesis of the viral proteins.