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用~3H-半乳糖掺入与软琼脂克隆培养相结合测CSA的方法,初步建立起对巨噬细胞产CSFs有促进作用的药物筛选模型。在用此模型对茯苓素的筛选中,我们发现茯苓素有效部分10150及从10150中分离出的单一组分10152在体外能增加LPS活化的小鼠腹腔巨噬细胞产CSFs的能力,且10152的增强作用有剂量依赖性关系。在体内实验中,腹腔注射10150能使小鼠血清中CSA增高,外周血白细胞水平上升。另外,用腹腔注射环磷酰胺造成的小鼠外周血白细胞减少模型,我们发现先期腹腔注射10150能预防由环磷酰胺引起的白细胞减少(白细胞下降幅度由56％减少到37％),而在每次腹腔注射环磷酰胺(25mg/(kg·d),连续四天)后1小时再腹腔注射10150(25mg/(kg·d),连续四天),能使白细胞下降幅度明显减少(白细胞下降幅度由53％减少到27％),且恢复时间提早2天,效果强于升白药阿魏酸钠。
Using 3H-galactose incorporation method combined with soft agar culture to detect CSA, a drug screening model for promoting the production of CSFs by macrophages was initially established. In the screening of Poria by this model, we found that Poria culin effector 10150 and single component 10152 isolated from 10150 can increase the ability of LPS-activated mouse peritoneal macrophages to produce CSFs in vitro and that 10152 Enhance the role of a dose-dependent relationship. In vivo experiments, intraperitoneal injection of 10150 can make mice serum CSA increased peripheral blood leukocyte levels. In addition, we found that intraperitoneal injection of 10150 prevented cyclophosphamide-induced neutropenia (reduction in leukocyte count from 56% to 37%) by intraperitoneal injection of cyclophosphamide-induced mouse peripheral leukopenia, whereas at each Intraperitoneal injection of cyclophosphamide (25mg / (kg · d) for 1 hour after intraperitoneal injection of 25 mg / (kg · d) for 10 days (25mg / (kg · d) for four consecutive days) significantly reduced the leukopenia (leukopenia Amplitude decreased from 53% to 27%), and the recovery time two days earlier, the effect is stronger than the sodium bromide sodium ferulate.