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目的 探讨心复康口服液改善心功能的作用机制。方法 通过腹腔注射阿霉素复制大鼠心衰模型，以电镜细胞化学方法，研究中药心复康口服液对大鼠心衰模型心肌 Ｃａ２＋ 定位及 Ｃａ２＋ － Ａ Ｔ Ｐａｓｅ 活性的影响。结果 阿霉素模型组心肌细胞内 Ｃａ２＋ 沉淀颗粒明显增多，高电子密度沉淀物大量沉着于肌膜内侧、线粒体膜及肌质中，肌浆网内以及肌丝周围沉淀颗粒较大，散在或聚集分布，有的连成丝团状。心复康组心肌细胞内 Ｃａ２＋ 沉淀颗粒较正常对照组多，较 Ａ Ｄ Ｒ 组显著减少，沉着颗粒主要见于线粒体和肌浆网内，密度较低。 Ｃａ２＋ － Ａ Ｔ Ｐａｓｅ 活性观察发现， Ａ Ｄ Ｒ 模型组心肌细胞 Ｃａ２＋ － Ａ Ｔ Ｐａｓｅ 反应较弱，沉淀物在肌膜和线粒体膜上较多。心复康治疗组心肌细胞 Ｃａ２＋ Ａ Ｔ Ｐａｓｅ 反应较正常对照组、 Ａ Ｄ Ｒ 模型组增强，反应物广泛分布于肌纤维膜、线粒体膜及肌浆网膜各处，呈细小园颗粒状，几乎连成线状围绕上述生物膜分布。结论 提示心复康口服液具有一定的钙拮抗作用，其机制可能是通过提高 Ｃａ２＋ － Ａ Ｔ Ｐａｓｅ 活性促进 Ｃａ２＋ 转运而发挥作用。
Objective To investigate the mechanism of Xinfukang oral solution in improving heart function. Methods Rat heart failure model was induced by intraperitoneal injection of doxorubicin, and the effects of Xinfukang oral liquid on Ca2+ localization and Ca2+-ATPase activity were studied by electron microscopy and cytochemistry. RESULTS: In the adriamycin model group, the Ca 2+ -precipitated granules in the cardiomyocytes were significantly increased. The high electron density deposits were abundant in the inner sarcolemma, mitochondrial membranes, and sarcoplasmic reticulum. The granules in the sarcoplasmic reticulum and around the myofilaments were larger, scattered or aggregated. Distribution, some even filamentous group. The concentration of Ca2+ precipitated in myocardial cells in Xinfukang group was higher than that in normal control group, which was significantly less than that in ADR group. The precipitated particles were mainly found in mitochondria and sarcoplasmic reticulum, and the density was lower. The activity of Ca2+-A T Pase was found to be weak in the Ca2+-A T Pase response in the ADR model group, and there were more deposits in the sarcolemma and mitochondrial membranes. Compared with the normal control group and the ADR model group, the cardiomyocyte Ca2+A T Pase response was increased in the Xinfukang-treated group, and the reactants were widely distributed in the muscle fiber membrane, mitochondrial membrane, and sarcoplasmic reticulum. Linearly distributed around the above biofilm. Conclusion It is suggested that Xinfukang Oral Liquid has a certain calcium antagonistic effect. The mechanism may be that it promotes Ca2+ transport by increasing Ca2+-A T Pase activity.