通痹灵对大鼠佐剂性关节炎滑膜细胞功能的影响

• 作 者: 陈纪藩 赵会芳 沈晓燕 李颂华 李迎敏 廖世煌 黄仰模 奥西秀树 邱联群 关彤 方永奇 刘晓玲
• 机 构: 广州中医药大学第一附属医院,日本岛根医科大学
• 出 处: 中国中西医结合杂志
• 发表日期: 1999年03期
• 关键词: 滑膜细胞 通痹 佐剂性关节炎 成纤维细胞增殖 细胞因子 细胞增殖 细胞培养 肿瘤坏死因子α 白细胞介素 原代培养

目的：研究通痹灵（ＴＢＬ）对佐剂性关节炎（ＡＡ）大鼠滑膜成纤维细胞增殖及滑膜细胞分泌白细胞介素１（ＩＬ１）、肿瘤坏死因子α（ＴＮＦα）和前列腺素Ｅ２（ＰＧＥ２）的影响。方法：采用组织块培养的方法获得滑膜成纤维细胞，检测原代培养大鼠滑膜细胞培养上清对滑膜成纤维细胞增殖反应的影响，以及滑膜细胞培养上清中ＩＬ１、ＴＮＦα的活性和ＰＧＥ２的含量。结果：ＴＢＬ可以明显抑制ＡＡ大鼠滑膜成纤维细胞增殖（Ｐ＜０００１），下调ＡＡ大鼠滑膜细胞分泌ＩＬ１、ＴＮＦα和ＰＧＥ２（Ｐ＜００１）；消炎痛对滑膜成纤维细胞的增殖具有明显的刺激作用（Ｐ＜０００１），虽能显著抑制ＡＡ大鼠滑膜细胞分泌ＰＧＥ２，但进一步上调了ＩＬ１、ＴＮＦα的产生（Ｐ＜００１）。结论：ＴＢＬ治疗ＲＡ的机理之一可能是通过下调滑膜细胞的分泌功能，使ＡＡ大鼠滑膜成纤维细胞的过度增殖恢复正常

Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblasts and synovial cells secreting interleukin-1 (IL1) and tumor necrosis factor α (TNFα) in rats with adjuvant arthritis (AA). Effects of prostaglandin E2 (PGE2). METHODS: Synovial fibroblasts were obtained by tissue culture method. The effects of primary cultured rat synovial cell culture supernatants on the proliferation of synovial fibroblasts were measured, and IL1 in synovial cell culture supernatant was detected. TNF-α activity and PGE2 content. Results: TBL could significantly inhibit the proliferation of synovial fibroblasts in AA rats (P<0001), and down-regulate IL1, TNFα and PGE2 secreted by synoviocytes of AA rats (P<001); The proliferation of synovial fibroblasts was significantly stimulated (P<0001). Although it could significantly inhibit the secretion of PGE2 in synoviocytes of AA rats, it further upregulated the production of IL1 and TNFα (P< 001). Conclusion: One of the mechanisms of TBL in the treatment of RA may be through the down-regulation of the secretory function of synovial cells, and the excessive proliferation of synovial fibroblasts in AA rats may return to normal.