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目的探讨阿魏酸钠(SF)对百草枯(PQ)中毒大鼠急性肺损伤的治疗作用。方法健康Wister大鼠54只,随机分为对照组6只,中毒组24只,SF治疗组24只。对大鼠一次性腹腔注射PQ15 mg/kg诱导急性肺损伤,染毒同时、染毒后4、24、48h腹腔注射SF(50mg/kg),分别于染毒后8、24、48、72h各组处死动物6只,测定大鼠血浆和肺组织匀浆中的超氧化物歧化酶(SOD)活力、丙二醛(MDA)及内皮素(ET)含量,并观察肺组织病理变化。结果大鼠染毒后8、24、48、72h血浆及肺组织中的SOD活力较对照组明显下降,差异有统计学意义(P<0.05),MDA及ET含量则明显高于对照组,差异有统计学意义(P<0.01)。与中毒组比较,SF治疗组血浆24、48、72h SOD活力[(13.63±1.85)、(12.46±1.65)、(11.87±1.83)U/mg]和肺组织8、24、48、72h中的SOD活力[(1.42±0.26)、(1.09±0.21)、(0.89±0.81)、(0.69±0.13)U/mg]明显上升,差异有统计学意义(P<0.01);各时点血浆及肺组织MDA及ET含量亦明显下降,差异有统计学意义(P<0.05或P<0.01)。结论SF可使PQ中毒致肺损伤的血浆肺组织SOD活力明显上升,使MDA及ET含量下降,能改善PQ中毒引起的损伤。
Objective To investigate the therapeutic effect of sodium ferulate (SF) on acute lung injury induced by paraquat (PQ) in rats. Methods Fifty-four healthy Wister rats were randomly divided into control group (n = 6), poisoning group (n = 24) and SF group (n = 24). Rats were injected intraperitoneally with PQ15 mg / kg to induce acute lung injury. At the same time, SF (50 mg / kg) was intraperitoneal injected at 4, 24, and 48 h after exposure, respectively. Six rats were sacrificed. The activities of superoxide dismutase (SOD), malondialdehyde (MDA) and endothelin (ET) in plasma and lung homogenate of rats were determined. The pathological changes of lung tissue were observed. Results After 8, 24, 48 and 72h exposure, the activity of SOD in plasma and lung tissue of rats was significantly lower than that of the control group (P <0.05), and the contents of MDA and ET were significantly higher than those of the control group There was statistical significance (P <0.01). Compared with the poisoning group, the activities of SOD in the plasma of the SF group were significantly higher at 24, 48, and 72h ([(13.63 ± 1.85), (12.46 ± 1.65), (11.87 ± 1.83) U / mg] (P <0.01). The activity of SOD in group A was significantly higher than that in group B (P <0.01) Tissue MDA and ET levels also decreased significantly, with statistical significance (P <0.05 or P <0.01). Conclusion SF can significantly increase the activity of SOD in the plasma of lung tissue induced by PQ poisoning, decrease the content of MDA and ET, and improve the damage caused by PQ poisoning.