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通过对体外传代培养的人血管起源平滑肌肉瘤细胞进行直接和脂质体Ｔｆｘ－５０包裹的人心钠素（ＡＮＦ）基因表达质粒ｐｃＤＮＡ３／ＡＮＦ转染，并以非同位素地高辛标记的ＲＮＡ分子探针对转染细胞中的ＡＮＦｍＲＮＡ水平进行ＲＮＡＤｏｔＢｌｏｔ分子杂交检测，同时利用放射免疫方法测定转染细胞培养上清中的ＡＮＦ含量。结果显示，与未经脂质体Ｔｆｘ－５０包裹的ｐｃＤＮＡ３／ＡＮＦ直接进行细胞转染及低浓度脂质体Ｔｆｘ－５０包裹的ｐｃＤＮＡ３／ＡＮＦ进行细胞转染相比，脂质体Ｔｆｘ－５０与ｐｃＤＮＡ３／ＡＮＦ以３∶１（荷质比）进行包裹，并当ｐｃＤＮＡ３／ＡＮＦ在细胞数为１０５达到２．０μｇ时，细胞中ＡＮＦｍＲＮＡ表达阳性，转染细胞培养上清中ＡＮＦ含量达到５８．０ｎｇ／Ｌ。表明通过脂质体Ｔｆｘ－５０介导可以提高ｐｃＤＮＡ３／ＡＮＦ的转染效率，并使细胞表达ＡＮＦ显著增加。这一研究结果为利用脂质体法转染人心钠素基因进行血管阻塞性疾病的基因治疗研究提供了一定的实验基础
The human vascular derived leiomyosarcoma cells cultured in vitro were directly transfected with the lipofection Tfx-50 human ANP gene expression plasmid pcDNA3 / ANF, and the non-isotopic digoxin-labeled RNA molecules were probed The level of ANF mRNA in transfected cells was detected by RNADotBlot hybridization, and the level of ANF in the supernatant of transfected cells was determined by radioimmunoassay. The results showed that compared with pcDNA3 / ANF without liposome Tfx-50 directly transfected cells and low concentration of liposome Tfx-50 encapsulated pcDNA3 / ANF cell transfection compared with liposome Tfx-50 and pcDNA3 / ANF was packaged in a 3: 1 ratio (mass-to-mass ratio), and when the number of pcDNA3 / ANF cells reached 105, the expression of ANFmRNA in the cells was positive. The ANF content in the supernatant of transfected cells reached 58.0ng / L. The results showed that the transfection efficiency of pcDNA3 / ANF was enhanced by the liposome Tfx-50, and the expression of ANF in cells was significantly increased. The results of this study provide some experimental basis for the gene therapy study of vascular obstructive disease by liposomes transfection of human atrial natriuretic peptide