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作者从LOU/C大鼠免疫细胞瘤中获得1R938F不分泌型骨髓瘤细胞,从感染1,500条曼氏血吸虫尾蚴5周的雌性LOU/C大鼠的脾脏中分离脾细胞,并与骨髓瘤细胞进行融合。血清为感染4～5周大鼠血清和不感染的正常大鼠血清。用有限稀释法稀释将产生抗曼氏血吸虫抗体的细胞于96孔微量板内,以正常BALB/C小鼠腹腔粘附细胞为饲养层进行克隆化培养。将培养8天的阳性克隆细胞皮下注射LOU/C大鼠,3周后取瘤肿块、匀化,获得杂交瘤细胞,注射于LOU/C大鼠,2周后取腹水,于-20℃保存备用。间接荧光抗体法按Ramalho-Pinto等
The authors obtained 1R938F non-secreting myeloma cells from LOU / C rat immunocytomas, isolated spleens from spleens of 5-week-old female LOU / C rats infected with 1,500 S. mansoni cercariae and performed with myeloma cells Fusion. Serum was serum from 4 to 5 weeks of infection and normal rat serum from no infection. By limiting dilution method to produce anti-human Schistosoma japonicum antibody-producing cells in 96-well microplate, normal BALB / C mice peritoneal adhesion cells for feeder layer cloning and culture. LOU / C rats were injected subcutaneously with positive clone cells for 8 days. After three weeks, the tumor masses were homogenized and hybridoma cells were obtained and injected into LOU / C rats. After 2 weeks, ascites fluid was stored at -20 ℃ spare. Indirect fluorescent antibody method according to Ramalho-Pinto and so on