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作者用一种特制的毛细微玻片代替载玻片,来进行形成空斑细胞(PFC)的检侧。这种微玻片的规格为0.22mm×4mm×10mm,其容量为88μl。使用时只需将微玻片的一端放入微孔板中配好的反应混合液内,由于毛吸作用,微玻片即可被充满。用石蜡封口,放37℃孵育1—2小时后,即可用低倍光镜或自动电子计数器计数结果。通过小鼠脾细胞PFC检侧实验表明,此方法重复性很好,与Gunningham玻片法比较,PFC/10~6脾细胞;SRBC免疫小鼠后不同时间脾细胞PFC
The authors used a specially made capillary slide instead of a slide to perform the detection of plaque cells (PFCs). The size of this microslide is 0.22 mm × 4 mm × 10 mm and its capacity is 88 μl. Just use one end of the microt slide in a microtiter plate with a good reaction mixture, as a result of capillary action, the microt slide can be filled. After sealing with paraffin and incubating for 1-2 hours at 37 ° C, the results can be counted with a low power optical microscope or an automatic electronic counter. The test of PFC in mouse spleen cells showed that this method was very reproducible. Compared with Gunningham slide method, PFC / 10 ~ 6 splenocytes were detected in spleen cells;