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目的:建立午餐肉中盐酸克伦特罗的SPE-HPLC检测方法。方法:用0.2 mol/L盐酸溶液超声提取样品中的盐酸克伦特罗,加入亚铁氰化钾和乙酸锌沉淀蛋白质、淀粉和脂肪等,MCX固相萃取小柱净化、富集,以Eclipse XDB-C18(4.6 mm×150 mm×8μm)为色谱柱,0.01 mol/L磷酸二氢钠溶液(pH=6):甲醇=65:35为流动相分离,流速为1.0 ml/min,用DAD检测,检测波长为210 nm。结果:在该色谱条件下,4.4min可达到基线分离,RSD为0.17%~0.24%,回收率为89.40%~97.62%。结论:该方法简便、准确,可用于肉类食品安全与质量监控。
Objective: To establish a SPE-HPLC method for the determination of clenbuterol in lunch meat. Methods: Clenbuterol hydrochloride was extracted by sonication with 0.2 mol / L hydrochloric acid solution. Protein, starch and fat were precipitated by potassium ferrocyanide and zinc acetate. MCX solid phase extraction cartridges were purified and enriched with Eclipse XDB-C18 (4.6 mm × 150 mm × 8 μm) was used as the chromatographic column. The mobile phase was 0.01 mol / L sodium dihydrogen phosphate solution (pH = 6): methanol = 65:35. The flow rate was 1.0 ml / Detection, detection wavelength of 210 nm. Results: Under the chromatographic conditions, baseline separation was achieved in 4.4 min with RSD of 0.17% -0.24% and recovery of 89.40% -97.62%. Conclusion: The method is simple and accurate and can be used for meat food safety and quality control.