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目的:研究P2X3受体在滥用氯胺酮致膀胱功能障碍患者膀胱组织中的表达及其临床意义,讨论P2X3受体在滥用氯胺酮致膀胱功能障碍中的作用。方法:采用免疫组织化学方法分别检测36例滥用氯胺酮致膀胱功能障碍患者(试验组)和36例正常患者(对照组)膀胱组织中P2X3、M2、M3受体的表达情况,利用全自动显微镜及图像分析系统对免疫组化染色结果进行图像采集分析,测量其平均灰度值,同时比较两组间P2X3、M2、M3受体表达的差异。结果:P2X3受体主要在膀胱黏膜移行上皮细胞表达,试验组P2X3受体表达明显增强,与对照组比较差异有统计学意义(P<0.01);而M2、M3受体表达与对照组比较差异无统计学意义(P>0.01)。结论:滥用氯胺酮致膀胱功能障碍患者膀胱组织中P2X3受体表达显著增强,而M2、M3受体并未异常表达,提示P2X3受体上调可能在滥用氯胺酮致膀胱功能障碍中发挥关键作用。
OBJECTIVE: To investigate the expression of P2X3 receptor in bladder tissue of patients with ketamine-induced bladder dysfunction and its clinical significance, and to explore the role of P2X3 receptor in the abuse of ketamine-induced bladder dysfunction. Methods: Immunohistochemistry was used to detect the expression of P2X3, M2 and M3 receptors in 36 cases of ketamine-induced bladder dysfunction (experimental group) and 36 normal controls (control group), respectively. Image analysis system for immunohistochemical staining results were image acquisition and analysis, to measure the average gray value, while comparing the two groups P2X3, M2, M3 receptor expression differences. Results: The P2X3 receptor mainly expressed in transitional cell of bladder mucosa. The expression of P2X3 receptor in experimental group was significantly higher than that in control group (P <0.01), while the expression of M2 and M3 receptors was significantly lower than that in control group No statistical significance (P> 0.01). CONCLUSION: P2X3 receptor expression was significantly increased in bladder tissues of patients with bladder dysfunction due to abuse of ketamine, but not M2 and M3 receptors, suggesting that up-regulation of P2X3 receptors may play a key role in the abuse of ketamine-induced bladder dysfunction.