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AIM To obserwe tb e effect of pentagastrin (G-5) on IL-1β induced inhibition of insulin secretion in newbornrat islet of Langerhans.METHODS Islets of Langerhans of 3- to 5-day-old rats were isolated by collagenase digestion. The isletswere maintained free-floating in culture medium RPMI-1640, containing 10% (V/V) calf serum, anddistributed randomly in 96-well plastic plates (6 wells in each group). There are 15 islets per well in 0.2 mLculture medium. The islets were kept at 37℃ in mixed gases of 5% CO2 and 95% humidified air for the timerequired by the experimental design. Three experiments were performed in this study. (①) IL-13 inducedinhibition of insulin secretion in isolated islets of Langerhans. (②) Effect of G-5 on IL-lβ induced inhibition ofinsulin secretion. ③ Effect of G-5 on the functional repair of islet B-cells inhibited by IL-1β. Accumulatedand glucose stimulated insulin secretion was measured by radioimmunoassay in all studies. Data are presentedas ~ ± s. Differences between groups were analyzed using the Student's t test. P