论文部分内容阅读
[目的 ]运用分子生物学技术分析虫卵基因序列鉴定并殖吸虫病类型。 [方法 ]先从并殖吸虫病患者痰中分离出虫卵 ,然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因 (ITS2 ) ,并直接用于测序从而获得该基因的核苷酸序列。同时 ,亦用同法分别从动物宿主粪便中分离出的卫氏并殖吸虫和斯氏狸殖吸虫虫卵中获得ITS2基因序列作为DNA参照分析。此外 ,本文也对从患者痰中分离出的虫卵进行详细的形态学特征描述分析。 [结果 ]来自患者的虫卵ITS2基因序列与参照的卫氏并殖吸虫虫卵的基因序列 10 0 %一致 ,而与来自斯氏狸殖吸虫虫卵的基因序列只有 92 %核苷酸相同。此外 ,从形态学上讲 ,来自患者的虫卵形态特征更与卫氏并殖吸虫虫卵相似。 [结论 ]通过基因序列分析 ,可确诊患者所患的是卫氏并殖吸虫病。
[Objective] The research aimed to identify the genus Paragonimiasis by using molecular biology techniques. [Method] The eggs were isolated from sputum of patients with paragonimiasis, and then the complete ribosomal DNA second spacer gene (ITS2) was amplified by PCR and sequenced directly to obtain the nucleus of the gene Nucleotide sequence. In the meantime, the ITS2 gene sequence was also obtained from the Paragonimus westermani and Parsnovivitum isolates, which were isolated from feces of animal hosts by the same method, respectively, for DNA reference analysis. In addition, detailed morphological characterization and analysis of the eggs isolated from sputum of patients was also performed in this paper. [Results] The ITS2 gene sequence of the eggs from patients was consistent with that of the reference Paragonimus westermani eggs, and only 92% of the nucleotide sequences of the genes from the eggs of the eggs of Sarabzonehs were. In addition, morphologically, the morphology of the egg from the patient is more similar to the Paragonimus wirtei egg. [Conclusions] Paragonimus westermani can be diagnosed by gene sequence analysis.