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为阐明放射性肺纤维化的病变规律和发生机理提供形态依据，应用图像分析技术，对放射性肺纤维化形成过程中肺间质、肺泡腔、肺内基质弹力纤维含量、肺内肥大细胞嗜碱性颗粒含量及肺内成纤维细胞胶原ｍＲＮＡ含量进行了定量测定。结果显示，随照射时间延长，肺泡壁渐增厚、肺间质所占面积增大，肺泡腔进行性缩小。弹力纤维于照后１个月时即见增多持续至６个月，而于照射后９－１２个月近于正常。肥大细胞嗜碱性颗粒含量于照射１、２个月明显减少，３－９个月恢复近正常水平。肺内成纤维细胞胶原ｍＲＮＡ含量于照射后每个时间点均有不同程度增加。表明放射性肺纤维化病变是个动态发展过程；肺内肥大细胞可能参与了纤维化的形成；肺内胶原的转录增强可能是放射性肺纤维化形成的关键步骤之一。
In order to elucidate the regularity of the pathological changes and mechanism of radiation-induced pulmonary fibrosis, the image analysis technique was used to study the changes of pulmonary interstitial, alveolar space, contents of elastic fibers in the lung, basophilicity of mast cells in lung The content of granule and collagen mRNA in lung fibroblasts were quantitatively determined. The results showed that with the extension of irradiation time, alveolar walls gradually thickening, the area occupied by interstitial lung increased alveolar contraction. Stretch fiber at 1 month after irradiation that see the increase continued for 6 months, and 9-12 months after irradiation nearly normal. Mast cell basophilic granule content significantly decreased at 1 and 2 months of irradiation, returning to normal levels 3-9 months. Pulmonary fibroblast collagen mRNA levels increased at different time points after irradiation. It is indicated that the pathological changes of radiation-induced pulmonary fibrosis are a dynamic process. The intra-lung mast cells may be involved in the formation of fibrosis. The enhanced transcription of collagen in the lung may be one of the key steps in the formation of radiation-induced pulmonary fibrosis.