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目的探讨缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)基因多态性与中国四川地区汉族人群宫颈癌易感性、临床分期及组织病理学特征间的相关性。方法采用聚合酶链反应-限制性片段长度多态性分析方法,检测该地区97例宫颈癌和117例正常宫颈组织HIF-1αC1772T位点基因型的分布。结果宫颈癌组HIF-1αC1772T位点T等位基因频率高于对照组(P<0.01);T/T和T/C基因型携带者患宫颈癌风险增加。HIF-1αC1772T位点各基因型在不同临床分期及组织病理学特征的宫颈癌患者间分布差异无统计学意义(P>0.05)。结论①HIF-1αC1772T位点T等位基因可能是四川地区汉族女性罹患宫颈癌的分子遗传学标记;②HIF-1αC1772T位点SNP可能与四川地区汉族女性宫颈癌进展无关。
Objective To investigate the relationship between hypoxia inducible factor-1α (HIF-1α) gene polymorphism and cervical cancer susceptibility, clinical stage and histopathological features in Han nationality in Sichuan province, China. Methods The distribution of HIF-1αC1772T locus in 97 cases of cervical cancer and 117 cases of normal cervical tissue was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequency of T allele of HIF-1αC1772T site in cervical cancer group was higher than that in control group (P <0.01). The risk of cervical cancer was increased in T / T and T / C genotype carriers. There were no significant differences in the distribution of HIF-1αC1772T genotypes between cervical cancer patients with different clinical stages and histopathological features (P> 0.05). Conclusion ①T allele of HIF-1αC1772T locus may be a molecular genetic marker of cervical cancer in Han women in Sichuan province. ② The SNP of HIF-1αC1772T site may not be related to the progression of cervical cancer in Han women in Sichuan.