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目的:探讨南海常见的皱瘤海鞘、冠瘤海鞘和大洋纵列海鞘醇提取物对体外HepG2 2.215细胞的抗乙肝病毒作用。方法:将三种海鞘醇提取物以不同浓度作用于培养的HepG2 2.215细胞,再检测培养液中HBsAg和HBeAg滴度。结果:三种海鞘醇提取物的浓度大于1 g/L时对HBsAg和浓度大于0.5 g/L时对HBeAg的分泌具有明显的抑制作用;皱瘤海鞘在抗病毒效果相同时,其细胞毒性较其他两种海鞘低。结论:海鞘醇提取物对HepG22.215细胞有显著的抗乙肝病毒作用,以皱瘤海鞘醇提取物综合效果较好,是抗乙肝新药研究的较好原材料。
OBJECTIVE: To investigate the anti-hepatitis B virus (HBV) activity of HepG2 2.215 cells in vitro, which is commonly used in the South China Sea. Methods: Three kinds of scutellarin extracts were exposed to HepG2 2.215 cells at different concentrations, and the HBsAg and HBeAg titers in the culture fluid were measured. Results: When the concentrations of three esculin extracts were more than 1 g/L, the secretion of HBeAg was significantly inhibited when the concentration of HBsAg was more than 0.5 g/L. The cytotoxicity of kinins was the same when the anti-virus effect was the same. The other two are low in ascidians. Conclusion: The scutellarin extract has a significant anti-hepatitis B virus effect on HepG22.215 cells. The combined effect of the sclerotherapy sclerotherapy extract is better. It is a good raw material for the study of anti-hepatitis B new drugs.