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研究目的探讨原发性肾小球疾病与外周血肿瘤坏死因子水平变化的关系。研究设计病例对照研究。患者和其他参与者急性肾炎患者7例，肾病综合征9例，对照组30例，均为正常儿童。处理方法所有患者和参与者均于清晨取空腹静脉血，分离血浆-20℃保存待检。检测和主要结果采用L929细胞生物学活性法，经重组人TNF（Sigma）校准（1U＝1．67pg），并经抗TNF血清中和试验证实。对照组，肾炎组、肾病组TNF水平分别为133．33±146．44、468．57±267．05，613．33±387．81U／ml，痊愈好转后复查与治疗前对照，TNF水平分别为271．11±201．52、595．0±341．88U／ml，观察组与对照组及治疗前后相比均有显著性差异。结论肾脏病患儿外周血TNF水平显著高于正常健康儿童，而且TNF水平的增高与肾功能损伤和病情密切相关，提示应用抗TNF血清治疗原发性肾小球疾病可能有效。
Objective To investigate the relationship between primary glomerular diseases and tumor necrosis factor levels in peripheral blood. Study design Case-control study. 7 patients with acute nephritis, 9 patients with nephrotic syndrome and 30 patients with control group were all normal children. Treatment Methods All patients and participants were taken fasting venous blood in early morning, plasma was separated and stored at -20 ℃ pending. Detection and main results were confirmed by recombinant human TNF (Sigma) (1U = 1.67 pg) using the L929 cell biological activity assay and confirmed by anti-TNF serum neutralization assay. The levels of TNF in control group, nephritis group and nephropathy group were 133.33 ± 146.44, 468.57 ± 267.05 and 613.33 ± 387.81U / ml, respectively. 271.11 ± 201.52,595.0 ± 341.88U / ml, the observation group compared with the control group and before and after treatment were significantly different. Conclusion The level of TNF in peripheral blood of children with nephrotic syndrome is significantly higher than that of normal healthy children, and the increase of TNF level is closely related to renal function damage and disease, which suggests that the treatment of primary glomerular disease with anti-TNF serum may be effective.