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目的考察余甘子醇提物体外对人胃癌AGS细胞的生长抑制和诱导凋亡的作用。方法采用MTT法、细胞集落实验、Hoechst染色分析、Annexin-V-FITC/PI等方法对经过余甘子醇提物体外作用的AGS细胞进行观察和检测。结果AGS细胞经0.05,0.1,0.15 mg·m L?1余甘子醇提物作用后,各组细胞形态均发生不同程度的变化,并呈现浓度-时间依赖性;细胞集落实验表明余甘子醇提物抑制肿瘤细胞的集落形成率;Hoechst染色分析、Annexin V/PI实验表明余甘子醇提物能够促进肿瘤细胞的凋亡。结论余甘子醇提物在体外对人胃癌AGS细胞具有良好的抑制生长和诱导凋亡的作用。
Objective To investigate the effect of alcohol extract of Phyllanthus emblica on human gastric cancer AGS cell growth inhibition and induction of apoptosis. Methods MTT assay, colony assay, Hoechst staining assay and Annexin-V-FITC / PI assay were used to observe and detect AGS cells treated with alcohol extract. Results After AGS cells were treated with 0.05, 0.1 and 0.15 mg · mL -1 of amyl alcohol, the cell morphology of AGS cells varied in different degrees and showed concentration-time dependence. The results of cell colony assay showed that the alcohol extract Hoechst staining analysis and Annexin V / PI assay showed that the ethanol extract of Phyllanthus emblica could promote the apoptosis of tumor cells. Conclusions Alcohol extract of Phyllanthus emblica has good inhibitory effect on AGS cells and induces apoptosis of human gastric cancer cells in vitro.