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本实验采用幼年雄性大鼠睾丸Leydig细胞离体单层培养法,研究hCG对Leydig细胞睾酮和cAMP生成的影响,并探讨了不同剂量X线对Leydig细胞辐射效应的发生机理。实验结果表明,hCG能明显促进Leydig细胞睾酮和cAMP生成。细胞预培养24h接受不同剂量X线照射,停照后加入外源性hLCG(0.05IU/ml)继续培养48h,小剂量辐射(25～250mGy,剂量率12.5mGy/min)在25,50mGy剂量组引起Leydig细胞对hCG反应性增高,睾酮生成量明显高于对照组,细胞内cAMP含量也发生相应变化;大剂量辐射后(0.5～8.0Gy,剂量率0.5Gy/min),Leydig细胞在各剂量组对hCG反应性均降低,睾酮生成量和cAMP含量变化一致,并与照射剂量呈线性负相关。
In this experiment, the effect of hCG on testosterone and cAMP production in Leydig cells was studied using a single-layer culture of testis Leydig cells in young male rats, and the mechanism of radiation effects of different doses of X-rays on Leydig cells was investigated. The experimental results show that hCG can significantly promote testosterone and cAMP production in Leydig cells. The cells were pre-incubated for 24 h and received different doses of X-rays. After stopping irradiation, exogenous hLCG (0.05 IU/ml) was added to continue the culture for 48 h. Low-dose radiation (25-250 mGy, dose rate 12.5 mGy/min) was administered at 25,50 mGy. Caused by Leydig cells increased hCG reactivity, testosterone production was significantly higher than the control group, the corresponding changes in intracellular cAMP content; after high-dose radiation (0.5 ~ 8.0Gy, dose rate 0.5Gy/min), Leydig cells in each dose The reactivity of hCG in the group was decreased, and the change of testosterone production and cAMP content was consistent, and it was linearly and negatively correlated with the irradiation dose.