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作者以价廉的固体三N培养基和其他易得材料改进了Evans和Smith(1986)的原虫克隆技术。具体方法为:取内径14mm、长约10mm的一段玻管,垂直置于载玻片上并用抗热环氧树脂粘接,制成观察悬滴标本的简易湿盒。将原虫克隆放在直径6cm、盛有10ml固体三N培养基的培养皿内培育。配置三N培养基时,在每升水内加10gBacto-琼脂(Difco),6g NaCl以及10％的柠檬酸盐抗凝兔血。培养皿于48小时内使用以防止干燥。因在无菌条件下操作,故不加抗菌
The authors improved the protozoal cloning technique of Evans and Smith (1986) with inexpensive solid Tris-N media and other readily available materials. The specific method is as follows: Take a glass tube with an inner diameter of 14 mm and a length of about 10 mm, which is placed vertically on a glass slide and bonded with a heat-resistant epoxy resin to make a simple wet box for observing the hanging drop specimen. The protozoal clones were placed in a Petri dish of 6 cm in diameter and filled with 10 ml of solid Tris-N medium. When configuring triple N media, add 10 g Bacto-agar (Difco), 6 g NaCl, and 10% citrated anticoagulated rabbit blood per liter of water. Petri dishes are used within 48 hours to prevent drying. Because of the aseptic conditions, it is not anti-bacterial