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[目的]了解光照强度在黄芩Scutellaria baicalensis Georgi种子萌发过程中的初生与次生代谢规律。[方法]光合色素、可溶性糖、苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟化酶和查尔酮合成酶(CHS)活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]黄芩种子在萌发过程中对光照强度不敏感,黄芩种苗的生长对光照强度是非常敏感的,光合色素、可溶性糖、苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟化酶和查尔酮合成酶(CHS)活性均随着光照强度增强而增加,其次生代谢物质也呈现出相同的变化趋势。[结论]光照强度促进了光合色素的形成,进而促进了初生代谢物质、关键酶活性和次生代谢物质的合成,因此我们可以通过调节光照强度来提高黄芩药材的质量。
[Objective] The research aimed to understand the primary and secondary metabolism of light intensity during the seed germination of Scutellaria baicalensis Georgi. [Method] The photosynthetic pigments, soluble sugar, phenylalanine ammonia - lyase (PAL), cinnamic acid 4 - hydroxylase and chalcone synthase (CHS) activity were determined by UV spectrophotometry. Secondary metabolites were determined by high performance liquid chromatography. [Result] Scutellariae was insensitive to light intensity during germination. The growth of Scutellaria baicalensis seedlings was very sensitive to light intensity. Photosynthetic pigments, soluble sugar, PAL, The activities of hydroxylase and chalcone synthase (CHS) increased with the increase of light intensity, and the secondary metabolites showed the same trend. [Conclusion] The light intensity promoted the formation of photosynthetic pigments and promoted the metabolism of primary metabolites, key enzymes and secondary metabolites. Therefore, we could improve the quality of Radix Scutellariae by adjusting light intensity.