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目的:观察牛心柿叶多糖对链脲佐菌素致糖尿病小鼠降血糖作用。方法:建立糖尿病小鼠模型,随机分为模型组、二甲双胍阳性组及牛心柿叶多糖低、中、高剂量组。ig给予牛心柿叶多糖0.3,0.6,1.2 g·kg-1,阳性组给予二甲双胍0.32g·kg-1,连续灌胃15 d。末次给药后葡萄糖氧化酶法测定空腹血糖(FBG);比色法测定血清中游离脂肪酸(FFA)和谷胱甘肽(GSH)水平及肝脏组织中总超氧化物歧化酶(T-SOD)活性和丙二醛(MDA)含量;Western blot法检测胰脏胰岛素受体底物-1(IRS-1)和组织的胰岛素受体(INSR)的表达。结果:与模型组比较,牛心柿叶多糖有效降低糖尿病小鼠的血糖(P<0.05);升高血清中GSH水平,同时降低FFA水平(P<0.05);提高肝组织中T-SOD水平,同时减少MDA含量(P<0.05);上调胰脏组织中IRS-1,INSR的蛋白水平(P<0.05)。结论:牛心柿叶多糖发挥有效的降血糖作用,机制可能与其对糖尿病小鼠肝脏组织脂质过氧化损伤的保护作用以及改善胰脏组织中INS信号转导通路有关。
Objective: To observe the hypoglycemic effect of Polysaccharide from Taxus persicae on streptozotocin - induced diabetic mice. Methods: Diabetic mouse model was established and randomly divided into model group, metformin positive group and polysaccharide of pullulan, low, medium and high dose group. ig given polysaccharide 0.3, 0.6, 1.2 g · kg-1, the positive group given metformin 0.32g · kg-1, continuous gavage for 15 days. Fasting plasma glucose (FBG) was measured by glucose oxidase method after the last administration. The levels of free fatty acids (FFA) and glutathione (GSH) and total superoxide dismutase (T-SOD) Activity and malondialdehyde (MDA) contents were measured. The expression of insulin receptor substrate-1 (IRS-1) and insulin receptor (INSR) in pancreatic tissues were detected by Western blot. Results: Compared with model group, polysaccharide of Pulsatilla chinensis effectively reduced blood glucose in diabetic mice (P <0.05), increased serum GSH level, reduced FFA level (P <0.05), and increased T-SOD level in liver tissue (P <0.05). The protein levels of IRS-1 and INSR in pancreatic tissue were increased (P <0.05). CONCLUSION: Polysaccharide from Pulsatilla chinensis (Persimmon) has an effective hypoglycemic effect, which may be related to its protective effect on lipid peroxidation injury in liver tissues of diabetic mice and the improvement of INS signal transduction pathway in pancreatic tissues.